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Tauseef R. Butt, Ph.D.
[Update My Profile]
LifeSensors Inc.
Office: Great Valley Corp Center Email: butt@lifesensors.com
Phone: 610.644.8845 x300 Fax: 610.644.8616
Personal Web Page: Click Here to Visit Personal Web Page
Research Keywords:
Functional Protein Chips, biosensors, Structural Genomics, yeast genetics technology platform, nuclear receptor, signal transduction mechanisms, gene transcription regulation, fiber optics as means to detect signals from cell-based sensors, human nuclear receptors as nanomachines, drug discovery and diagnostics, drug target genes.
Biosketch:
Positions and Employment
2002 – Present President, LifeSensors Inc., Malvern, PA
1996 – Present VP of R&D, LifeSensors Inc., Malvern, PA
2000 - Present Adjunct Professor, Dept of Biomedical Engineering, Drexel University, Philadelphia, PA
1991 – 2000 Adjunct Professor, Dept. of Biochemistry & Biophysics, University of Pennsylvania Medical School
1988 – 1995 Asst. Director, Dept. of Molecular Virology & Host Defense, SmithKline Beecham Pharmaceuticals
1985 – 1988 Sr. Investigator, Dept. of Molecular Pharmacology, SmithKline & French Pharmaceuticals
1982 – 1984 Assoc. Sr. Investigator, Dept. of Molecular Pharmacology, SmithKline & French Pharmaceuticals
1980 – 1982 Staff Fellow, NIDDK, National Institutes of Health, Bethesda, MD
1977 – 1980 Post-doctoral Associate, Dept. of Biochemistry, Georgetown Medical School, Washington, D.C.
Education:
B.Sc., University of Dundee, Scotland, Biochemistry, 1972
M.Sc., University of Dundee, Scotland, Biochemistry, 1973
Ph.D., University of Glasgow, Scotland, Molecular Biology, 1977
Active Research Projects:
Biosensors for drug discovery and diagnosis
Development gene expression and protein production technologies
Use of Yeast genetics to capture human gene function
Human nuclear receptors and innovative drug targets
Publications:
Selected Peer-Reviewed Publications (From a total of 79 Publications and six patents)
1. Marbelstone, J. Edavettal, S., Lim, Y., Lim, P., Zuo, X., Butt, T. R. (2006) Comparison of SUMO Fusion Technology with Traditional Gene Fusion System: Effects of Recombinant Protein Expression and Purification. Protein Science. 15: 182-189
2. Arnold, JJ, Bernal, A, Uche, U, Sterner, DE, Butt, TR, Cameron, CE, and Mattern, MR. (2006) SUMO isopeptidase assay based on poliovirus RNA polymerase activity. Analytical Biochem., (in press).
3. Butt, T. R., Edavettal S., Hall, J. and Mattern M. R (2005) SUMO Fusion Technology for Difficult to Express Proteins. Protein Expression and Purification. 43: 1-9
4. Zuo, Xun., Mattern, M.R., Tan, R., Li, S., Hall, H., Sterner, D. E., Shoo, J., Lim, P., Sarafianos, S. G., Kazi, L., Navas-Martin., Weiss, S. R. and Butt, T. R. (2005) Expression and Purification of SARS Coronavirus Proteins Using SUMO-Fusions. Protein Expression and Purification. 42: 100-110
5. Zuo, Xun., Li, Shuisen., Hall John,. Mattern, Michael R., Tran, Tran., Shoo, Joshua., Tan, Robin., Weiss, Susan and Butt T. R. (2005) Enhanced expression and purification of membrane proteins by SUMO fusion in Echerichia coli. J of Structural and Functional Genomics. 6: 103-111.
6. Malakhov, Michael P., Malakhova, Oxana A., Drinker, Mark, Weeks, Stephen, and Butt, T. R. (2004) SUMO fusion and SUMO-specific protease for efficient expression and purification of proteins. Journal of Structural and Functional Genomics 5: 75-86
7. Butt, T.R., Tran, H.T., Choi, J-W., Cho J.H., Ahn, C.H. (2001) A disposable cell-based Biochip for the detection of hormones and drugs. Micro Total Analysis System 2001. J.M. Ramsey and A van den Berg (Ed). Kluwer Academic Publisher , pp 83-84
8. Tran, H.T., Askari H., Shaaban S., Price L., Palli S R., Dhadialla T.S., Carlson G.R., Butt, T.R. (2001) Reconstructionof ligand-dependent transactivation of Choristoneura fumiferana Ecdysone Receptor in yeast. Mol Endocrinology 15: 1140-1153.
9. Tran, H.T., Shabaan, S., Mateen, M., Marsh J., Askari H., Schwartz, M.L., Butt, T.R. (2001) Requirement of co-factor for the ligand-mediated activity of the insect ecdysteroid receptor in yeast. J Mol Endocrinology 27:191-209.
10. Anafi, M, Yang, Y-F., Berlev, N.A., Berger, S.L. and Butt, T.R., and Walfish, P.G. (2000) GCN5 and ADA adapter proteins regulate T3/GRIP coactivator dependent gene activation by human thyroid hormone receptor. Mol Endo 14: 718-732.
11. Breithofer, A., Grauman, K., Scicchitano, M. S., Karathanasis, S. K., Butt, T. R. and Jungbauer, A. (1999) Regulation of human estrogen receptor by phytoestrogens in yeast and human cells. J of Steroid Biochemistry and Mol Biology 67: 421-429.
12. Butt, T.R. and Chen, D.J. (1999). Reconstruction of mammaliam nuclear receptor function in Saccharomyces cerevisiae. Julian Davies, Charles Harshberger (Ed.) Manual of Industrial Microbiology and Biotechnology, 2nd Edition, Section V. American Society for Microbiology, Washington, DC, pp. 527-538.
Patents:
1. Butt, T. R., Bernal A(2004) Methods for Assessing Isopeptidase Activity. US Patent Application No. 60/580,900
2. Butt, T. R., Malakhova O.A and Malakhov M. P (2003) Methods and composition for protein expression and purification. US Patent Application, 60/482817 and PCT.
3. Butt, T.R., Weeks, S.D., Tran, H.T., Malakhova, O.A., and Malakhov, M.P. (2002) Methods and compositions for protein expression and purification. International Patent Application No. PCT/US03/00436.
4. Tran, H.T., Askari, H., Schwartz, M. and Butt, T.R. (2001) Methods and composition for identifying ligands for nuclear receptors. International Patent Application No. PCT/US01/05429.
5. Butt, T.R. and Tran, H.T. (2001) Biosensors for identifying gender at embryonic stage. International Patent Application No. PCT/ US02/12590.
6. LaBean, T.H. and Butt, T.R. (1997). Materials and methods for producing random peptide libraries, using ubiquitin gene fusion. US Patent 5,656,467.
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