BIOMED Home >> | Who We Are | Faculty | Research | Undergraduate Program | Graduate Programs | Students | Alumni  | Contact Us

Print friendly version of this event. Mail this event to a friend.


Master's Thesis Defense - The Development of Polysaccharide-based Nanoparticles for the Prevention of Foam Cell Formation during Atherosclerosis

Master's Thesis Defense - Age- and Subject-specific Finite Element Modeling of the Adolescent Thoracic Spine for Scoliosis Research

Master's Thesis Defense - Strategies to Enhance Drug Loading and Functionality in Polymeric Ultrasound Contrast Agents

Ph.D. Research Proposal - Modulation and Use of the Medial Plantar Nerve Reflex in the Treadmill-Locomoting and Air-Stepping Spinal Cat

Ph.D. Thesis Defense - Non-thermal Dielectric Barrier Discharge (DBD) Treated Phosphate Buffer Saline (PBS): Mechanisms of Bacterial Inactivation and Cellular Toxicity

Ph.D. Thesis Defense - The Role of microRNAs in Pain

EVENTS Archive
Ph.D. Research Proposal - Analysis of the Degree and the Mechanism of Airborne Bacterial Inactivation using Dielectric Barrier Discharge
Date: August 20, 2010
Time: 3:00 PM
Location: Bossone Research Enterprise Center, Room: 709

Nachiket Vaze
Advisors: Alexander Fridman, Ph.D. and Kambiz Pourrezaei, Ph.D.

Non-thermal Plasma technology has emerged as a very attractive technology for sterilization treatment. Plasma is the fourth state of matter. It is defined as ionized gas produced by electrical discharge. There are various configurations of producing plasma. These different types of plasmas have a variety of applications. Non-thermal plasma is the type of plasma where the ionized gas is at room temperature, while the high energy electrons produced by the discharge produce reactive species from impact of the high energy electrons with neutral gas molecules.

This study investigates the effectiveness and mechanisms of sterilization using the Dielectric Barrier Discharge (DBD) plasma. An experimental setup was constructed for studying the effect of DBD on a bacterial aerosol (bioaerosol). The bacterium used in these experiments was the Escherichia coli bacterium. The effectiveness of the DBD setup was demonstrated by batch treatment of the bioaerosol. After a single exposure to DBD discharge, ~5log reduction was observed in the concentration of viable airborne bacteria. The different DBD treatment systems have been developed for inactivation studies. These results have led to the investigation into the mechanism of inactivation of airborne bacteria by DBD with application to bioaerosol decontamination.

DBD produces Ultraviolet Radiation, Ozone and other Reactive Oxygen Species (ROS) such O2-, OH-, O2(1Δg) etc. The experiments with pure Ozone have demonstrated that it Ozone produced by the plasma alone cannot be the cause of inactivation. The UV measurement also indicates that UV produced by plasma has too low intensity to produce such rapid inactivation. The focus has then shifted to ROS. Experimental evidence suggests a working hypothesis that bacteria are inactivated through the action of ROS, with charges produced by DBD playing a role. The study employs methods such as survival of gene suppression mutants, flow cytometry, and fluorescence studies to determine the effect of DBD plasma on the bacteria.


The Bossone Research Enterprise Center is located at the corner of 32nd and Market Streets.

Phone 215.895.2215 | Fax 215.895.4983 | Email
Copyright 2013, Drexel University, All Rights Reserved.