Search
BIOMED Home >> | Who We Are | Faculty | Research | Undergraduate Program | Graduate Programs | Students | Alumni  | Contact Us

Print friendly version of this event. Mail this event to a friend.

CURRENT EVENTS...

Ph.D. Thesis Defense - Germline Sequence Analysis of Codon Bias and Somatic Mutational Diversity in Variable (V) Genes in the Immune Repertoire: Models for Cross Talk Between Mutation and Selection

Joint ECE-Biomed Seminar - Building Models of Cell Differentiation and Perturbation Directly from Microscope Images

Master's Thesis Defense - NeuroHub: Portable and Scalable Time Synchronization Instrument for Brain-Computer Interface and Functional Neuroimaging Research

Master's Thesis Defense - Analysis of the Hip Morphological Parameters and Comparison of Interference Patterns between Normal and Femoral Acetabular Impingement Patients

Ph.D. Research Proposal - Quantifying the Diversity of the Lymphocyte Receptor Repertoire

Seminar - Brain Plasticity in High Definition


EVENTS Archive
EVENT GALLERY Archive
NEWS & EVENTS Home
BIOMED Home
Master's Thesis Defense - Hetergoeneous Spatial Distribution of Purinergic Receptor P2Y2 in Microvessel Endothelial Cells
Date: July 9, 2007
Time: 9:00 AM
Location: Bossone Research Enterprise Center, Room: 702

Speaker(s):
Christin Alejnikov
Advisor: Kenneth A. Barbee, Ph.D.

Details:
This study details the investigation of key proteins in the nitric oxide production pathway in microvessels. Nitric oxide is an essential molecule in the prevention of disease states like atherosclerosis, hypertension, clotting disorders, and autoimmune disease. While large vessel endothelial cells are regularly studied for nitric oxide pathway proteins, little data has been produced on the distribution of these proteins in microvessels. Previous cell culture models have demonstrated heterogeneous calcium responses as part of the nitric oxide production pathway when microvessel cells are stimulated with ATP. Here we present the characterization of spatial heterogeneity of the purinergic receptor P2Y2 in rat adrenal medulla capillary cells, as well as the distributions of the microdomain caveolae (identified by caveolin-1) and endothelial nitric oxide synthase, in vivo.

Furthermore, we present a program used to analyze immunohistochemical images to quantify the expressio n of the proteins of interest within the endothelium. Immunohistochemical staining of these proteins was performed and analyzed for fluorescence intensity and distribution within the tissue’s endothelium. The Kolmogorov-Smirnov Test was employed to verify statistical significance of the experimental data from controls. The study reveals populations of endothelial cells that express clearly bimodal distributions of the purinergic receptor, indicating spatial heterogeneity. Caveolin-1 and endothelial nitric oxide synthase distributions are uniform and colocalized, as previous research indicates in other tissues.

Biosketch:

Directions:
The Bossone Research Enterprise Center is located at the corner of 32nd and Market Streets.

Phone 215.895.2215 | Fax 215.895.4983 | Email biomed@drexel.edu
Copyright 2013, Drexel University, All Rights Reserved.