Master's Thesis Defense - Detection of Cardiac Allograft Rejection Using Raman Spectroscopy
Date: April 13, 2007
Time: 2:00 PM
Location: Bossone Research Enterprise Center, Room: 702
Advisor: Chang Chang, Ph.D.
Heart transplantation is the last-resort treatment for patients with end-stage heart failure and its efficacy is limited by cardiac allograft rejection. Post-transplantation endomyocardial biopsy is the current gold standard for rejection surveillance. Severity of allograft rejection is graded histopathologically by staining the incised biopsy sample with haematoxylin & eosin (H&E). Current grading system is standardized by the International Society for Heart and Lung Transplantation (ISHLT) in 1990, with Grade-0 representing no observable rejection and Grade-4 being the most severe rejection.
The goal of this research is to ultimately replace this histopathological procedure with an optical diagnostic modality, namely Raman spectroscopy. Such optical detection method eradicates the need for tissue removal and as a result is expected to reduce the mortality and morbidity associated with tissue incision. Here we have successfully demonstrated Raman spectroscopy’s ability to distinguish between Grade-0 and Grade-2 cardiac allograft rejections based on the observed spectral differences. Multivariate statistical analysis is also used to examine our current data set and its ability to cluster large numbers of spectral data into two target groups is successfully demonstrated. We have therefore shown here a potentially clinically-feasible in vivo optical detection method for cardiac allograft rejection.
The Bossone Research Enterprise Center is located at the corner of 32nd and Market Streets.