Search
BIOMED Home >> | Who We Are | Faculty | Research | Undergraduate Program | Graduate Programs | Students | Alumni  | Contact Us

Print friendly version of this event. Mail this event to a friend.

CURRENT EVENTS...

Joint ECE-Biomed Seminar - Building Models of Cell Differentiation and Perturbation Directly from Microscope Images

Master's Thesis Defense - NeuroHub: Portable and Scalable Time Synchronization Instrument for Brain-Computer Interface and Functional Neuroimaging Research

Master's Thesis Defense - Analysis of the Hip Morphological Parameters and Comparison of Interference Patterns between Normal and Femoral Acetabular Impingement Patients

Ph.D. Research Proposal - Quantifying the Diversity of the Lymphocyte Receptor Repertoire

Seminar - Brain Plasticity in High Definition

Ph.D. Thesis Defense - Mechanisms of Axonal Pathology in the Context of Traumatic Brain Injury


EVENTS Archive
EVENT GALLERY Archive
NEWS & EVENTS Home
BIOMED Home
Masters Thesis Defense - Development of Automated Brightfield Imaging for Cryoplane Microscopy
Date: December 22, 2005
Time: 11:00 AM
Location: Queen Lane Campus, Room: 261

Speaker(s):
Pallavi Bakare
Advisor: Jonathan Nissanov, Ph.D.

Details:
Cryoplane Microscope (CM) is a device used to examine biological material at a macro level of magnification using brightfield imaging and at a macro/micro level of magnification using epifluorescence microscopy. On this system, a frozen tissue is cut using a cryostat and the exposed blockface surface is imaged macroscopically using a line scan camera triggered by a linear encoder and then by an area scan camera attached to a microscope for micro level resolution. The tissue moves horizontally in our CM while the knife descends vertically. All imaging devices are mounted on the knife housing and descended along with the knife and thus focus is maintained on the blockface surface. By cutting the tissue block, deeper tissue levels are revealed. Successive planes are obtained as a series to yield an aligned dataset.

A Virtual Instrument (VI) using LabVIEW was developed as part of this thesis. It includes subVIs for monitoring tissue position, controlling intensity of brightfield illumination and image acquisition. Each line of the line scanner was triggered by the VI using data from a linear encoder monitoring tissue position. The pitch of the acquired line scan images is 14 Ám and those provide anatomical context for the higher resolution, up to 0.7 Ám pitch, obtained with the epifluorescence microscopy.

CM is being used in a variety of applications. Two notable ones are fluorescent imaging of gene expression using GFP transgenics and mapping of neural connectivity using fluorescence tract tracers. In both settings, the developed brightfield imaging system in conjunction with developed systemic stains will provide the anatomical framework to aid in understanding of the fluorescent signal obtained with the epifluorescent microscope.

Biosketch:

Directions:
The Queen Lane Campus is located at 2900 Queen Lane, Philadelphia, PA. A shuttle is available from 33rd and Market Streets

Phone 215.895.2215 | Fax 215.895.4983 | Email biomed@drexel.edu
Copyright 2013, Drexel University, All Rights Reserved.