Thesis Defense - Fibronectin Adsorption to Polystyrene in Varied Electric Field Measured by Fluorescent Labeling
Date: August 1, 2000
Time: 1:00 PM
Location: Commonwealth Hall, Room: 410
Speaker(s):
Hanako Yamanaka, M.S. Candidate in Biomedical Engineering
Supervising Professor: Dr. Michele Marcolongo
Details:
Fibronectin Adsorption to Polystyrene in Varied Electric Field Measured by Fluorescent Labeling
Fibronectin (Fn) is a glycoprotein with a cell-binding site that allows adhesion of cells to biomaterial surfaces. Integration of tissue with implants and tissue engineering scaffolds (designed to grow and proliferate cells) is crucial to the success of certain orthopedic devices. Studies have shown increased cell adhesion with negatively charged surfaces and inhibition with positively charged surfaces. Here the effect of material surface charge on Fn adsorption was studied by application of an electric field to the Fn solution. Controlling Fn adsorption by material surface charge would allow engineering biomaterials with specific cell-adhesion capabilities.
At 54 V, Fn adsorption was inhibited for both positively and negatively charged tissue culture polystyrene (TCP). However, the inhibition appeared to be a result of denaturation of Fn. At lower voltages of 6 and 12 V, no change in Fn adsorption was observed for both positively and negatively charged surfaces. No correlation between material surface charge and Fn adsorption was found. This suggests material surface charge affects Fn activity by alteration of Fn conformation upon adsorption.
Fn adsorption to TCP was quantified using a fluorescent labeling technique developed for this study. The fluorescent labeling technique eliminated the disadvantages of current methods and showed accuracy and consistency equivalent to that of radiolabeling and enzyme immunoassays.
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